Amino Acids by HPLC

 

                                                                                                                                                      

 

Amino acids in physiological fluids are mostly derived from the metabolism of body proteins. The concentrations of individual amino acids in plasma and urine are relatively stable. Enzyme deficiencies in biochemical pathways can lead to an accumulation of specific amino acids, enabling recognition and diagnosis of several metabolic diseases. Primary amino acids were derivatized with o-phthalaldehyde 3-mercaptopropionic acid (OPA) and detected by a diode array detector.

 

Amino Acids by HPLC is a kit for the quantitative determination of the amino acids in serum.

 

Technical Specifications:

 

Principle:                                     Gradient HPLC (Reversed Phase)

 

Duration of chromatography:      30 minutes

 

Sample volume:                          0,2 mL serum

 

Flow rate:                                    0.5 mL/min

 

Detection:                                    338 nm

 

 

 Performance

 

Linearity:                                     5000 µmol/L

 

Detection Limit:                           0,1 µmol/L

 

Recovery:                                   90-99%

 

CV% intra batch:                        4.3-4,9%

 

CV% inter batch:                        4.4-5.1%