URINARY METABOLITES by HPLC

                                                                                                                                                      

 

 

Vanillylmandelic acid (VMA) is a principle urinary metabolite of  catecholamines epinenephrine and norepinephrine,  and homovanillic acid (HVA) is a principle metabolite of dopamine. HVA and VMA  are formed by the action of the anzymes catechol-O-methyl transferase and monoamine oxidase. Determination of VMA and HVA in urine is as useful tool in the diagnosis and maintenance  of tumors of neural crest (pheochromocytoma,  neuroblastoma, and ganglioneuroma). 5-hydroxyindoleacetic acid (5-HIAA) is the major metabolite of serotonin, formed by the action of monoamine oxidase. 5-HIAA is commonly used for the diagnosis of carcinoid tumors.

Urinary Catechoacids by HPLC is a kit for the quantitative simultaneous determination of vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), for diagnosis of pheochromocytoma, neuroblastoma, and carcinoid syndrome.

 

 

Urinary Metabolites by HPLC is a kit for the quantitative simultaneous determination of vanillylmandelic acid (VMA), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA), for diagnosis of pheochromocytoma, neuroblastoma, and carcinoid syndrome.

 

Technical Specifications:

 

Principle:                                         Isocratic HPLC method (Reversed Phase)

 

Duration of chromatography:            15 minutes;

 

Sample volume:                               500 µL urine

 

Flow rate:                                        1.1 mL/min.

 

Detection:                                        Electrochemical

 

 

 

 

 

 

 

 

 

 

 

 

 

Performance

 

Linearity:                              up to 30 mg/L

Detection Limit:                     0,01 mg/L

 

 

VMA

5-HIAA

HVA

Recovery

97-99%

96-99%

96-99%

CV (%)

Intra batch

4.3

4.2

4.5

CV (%)

Inter batch

6.2

6.3

5.9