URINARY CATECHOLAMINES by HPLC  (Reversed Phase)

                                                                                                                     

 

Catecholamines are useful for the body and are necessary for responding to fright or stress, and help the body react in 'fight or flight' situations. Catecholamines are generated in large amounts by the adrenal glands to react to stress. Dopamine, norepinephrine (noradrenaline), and epinephrine (adrenaline) are the main catecholamines. Catecholamines are known to increase mental alertness, muscle strength, breathing rate, blood pressure, and heart rate. Sometimes, tumours like pheochromocytoma can also increase the level of catecholamines in the urine and blood. This can lead to tremors, palpitations, headaches, excessive sweating, and high blood pressure. Since the catecholamine levels may fluctuate significantly during a 24-hours period, the determination from urine may detect excess production that is missed with the plasma testing.

 

Urinary Catecholamines by HPLC is done to check the level of the hormones dopamine, norepinephrine, and epinephrine, in the urine, for the diagnosis of pheochromocytoma.

 

Technical Specifications:                                                               

 

Principles:                                       Isocratic HPLC  (Reversed Phase)

 

Duration of chromatography:          20 minuties

 

Sample volume and type:               500  µL  of urine

 

Flow rate:                                        1 mL/min

 

Detection:                                        electrochemical

 

Number of test:                               100/kit

 

 

 

 

 

 

 

 

 

 

 

 

           

Performance

 

Linearity:                                        up to 500 µg/L

Detection Limit:                              0,05 µg/L 

 

Recovery: 

 

NE

E

DA

5HT

93-96%

92-99%

93-99%

93-96%

CV   (%)

Intra   batch

5.2

5.0

4.6

4.8

CV   (%)

Inter   batch

6.1

5.8

5.6

5.5