URINARY METANEPHRINES by HPLC

 

                                                                                                                                                      

 

 

 

Pheochromocytomas and other neuroendocrine tumors, paragangliomas and neuroblastomas, induce the abnormal secretion of catecholamines (epinephrine, norepinephrine, and dopamine). Metanephrine (MN), Normetanephrine (N-MN), and Methoxytyramine (3MT), (collectively referred to as metanephrines) are the 3-methoxy metabolites of epinephrine, norepinephrine, and dopamine respectively. The metanephrines are stable metabolites and are cosecreted directly with catecholamines by pheochromocytomas and other neural crest tumors. Despite the rarity and benignant of pheochromocytoma, if they are not treated, they can lead to more serious medical conditions. The dangers of uncontrolled severe hypertension and the very effective surgical treatment of this condition mean that diagnosis is important. Urinary or plasma catecholamines or metanephrines are commonly used to screen for pheochromocytomas prior to imaging.

 

Urinary Metanephrines by HPLC is a Kit for the quantitative simultaneous determination of Metanephrine (MN), Normetanephrine (N-MN), and Methoxytyramine (3MT), in urine.

 

Technical specifications:

 

Principle:                                         Isocratic HPLC method (Reversed Phase)

 

Duration of chromatography:          15 minutes;

 

Sample volume and type:                1 mL of urine

 

Flow rate:                                         1.0 mL/min

 

Detection:                                         Electrochemical

 

  

 

Performance

 

Linearity:                                          up to 500 µg/L

 

Detection Limit:                                0,05 µg/lL

 

  

Recovery

N-MN

MN

3MT

%

95-98

96-98

94-98

CV (%)

Intra batch

4.4

4.3

5.1

CV (%)

Inter batch

5.5

6.2

6.0