Rely-AMP MYCOPLASMAS

(multiplex pcr kit)

 

                                                                                                                                                                                     

 

M. hominis M genitalium and U. urealyticum colonise the genitourinary tract of humans. These organisms can cause urethritis and contribute to vaginitis  in women. They have been associated with sexually transmitted diseases and with chronic infections in people with weakened immune systems. Genitourinary infections associated with Mycoplasma hominis, Ureaplasma urealyticum Mycoplasma genitalium  include: nongonococcal urethritis, pelvic inflammatory disease, infertility, pyelonephritis and urinary calculi. Many studies show that mycoplasma leads to male infertility through decreasing sperm motility and invasion of the testes.

 

DETAILS

 

Rely-AMP MYCOPLASMAS is an in vitro polymerase chain reaction (PCR) assay for the direct, qualitative detection of Ureaplasma urealyticum,  Mycoplasma hominis e Mycoplasma genitalium from urogenital specimens. Rely-AMP MYCOPLASMAS provides all necessary reagents for reliable Ureaplasma urealyticum,  Mycoplasma hominis e Mycoplasma genitalium extraction andDNA detection.

The kit is designed for the detection of the Ureaplasma urealyticum,  Mycoplasma hominis e Mycoplasma genitalium DNA based on the amplification of the specific sequence of the U. urealyticum urease gene, M. hominis GAPDH gene, and M genitalium repetitive sequence element mgp-r2 and on the amplification of the sequence of the gene for RNasi by means of the Real time PCR.

 

TECHNICAL SPECIFICATIONS

 

 

 

Quantitative/qualitative

 

Qualitative

 

Extraction

 

Magnetic Resin

 

 

Technology

 

 

Multiplex Real time PCR

 

Sample type

 

Urine, cervical swabs, urethral swabs,  sperm,

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Internal control

 

 

RNasi gene

 

Positive control

 

Ureaplasma urealyticum,  Mycoplasma hominis e Mycoplasma genitalium plasmid DNA

 

Amplified region

 

U. urealyticum:urease gene, M. hominis:GAPDH gene, M genitalium: repetitive sequence element mgp-r2

 

 

Fluorophore

 

 

FAM - CY5 - HEX

 

Processing

 

Manual

Automated

 

Time to result

 

2 hours (after extraction)

 

 

 

 

PERFORMANCE

 

Diagnostic sensitivity:  94,2%

Diagnostic specificity: 99,9%

Analytical Specificity: no cross reazione

Analytical sensitivity: 10 copies plasmid

 

 

ORDERING INFORMATION

 

PRODUCT

CAT.NO.

COSTO

Rely-AMP MYCOPLASMAS  extraction and detection kit

MY50

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Rely-AMP  MYCOPLASMAS  detection kit

MY50D

Contact us