Rely-AMP HPV High Risk

 

                                                                                                                                                        

 

Human papillomavirus (HPV) is a DNA virus, that is capable of establishing productive infections only in keratinocytes of the skin or mucous membranes. HPV infections are believed to cause 96% of cervical cancer. More than 120 genotypes of HPV have been identified, of these, 14 genotypes have been classified as “high-risk HPV” (HR-HPV) because they can lead to cervical cancer.

 

 

High Risk HPV

Frequency Europe (%)

16

58,1

18

15,7

31

4,0

33

4,4

35

1,6

39

0,2

45

2,9

51

0,2

52

0,6

56

1,2

58

1,0

59

0,2

66

0,2

68

0,2

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

DETAILS

 

 

Rely-AMP HPV is an in vitro polymerase chain reaction (PCR) assay for the direct, qualitative detection of 14 HPV High Risk from clinical specimens. Rely-AMP HPV provides all necessary reagents for reliable detection of all High Risk HPV.

The kit is designed for the detection of the High Risk HPV DNA, based on the amplification of the specific sequence of the L1 region of Papilloma virus, by Real time PCR multiplex. The system has an internal control, based on amplification of Human RNase. Furthermore Rely-AMP HPV allows the genotyping of the HPV 16 and HPV 18.

 

TECHNICAL SPECIFICATION

 

Quantitative/qualitative

Qualitative 

Extraction

Magnetic Resin 

Technology

Multiplex Real time PCR 

Sample type

Cervical swabs in Medium PreservCyt 

Thermal cycler

Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers 

Internal control 

RNasi gene

Positive control

HPV plasmid DNA  

Amplified region

L1 

Fluorophore 

FAM - HEX - CY5

Processing

Manual

Automated 

Time to result

2 hours (after extraction) 

  

PERFORMANCE

 

Diagnostic sensitivity:  90%

Diagnostic specificity: 98%

Analytical Specificity: no cross reaction

Analytical sensitivity:

 

High Risk HPV

Copies/ml

16

300

18

500

31

500

33

500

35

500

39

500

45

300

51

300

52

1500

56

600

58

600

59

600

66

1500

68

1500

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

ORDERING INFORMATION

 

PRODUCT

CAT.NO.

 PRICE

Rely-AMP HPV

extraction and detection kit

 HPV HR50

 Contact us

Rely-AMP  HPV

detection kit

  HPV HR50D 

Contact us