Rely-AMP MTHFR
Hyperhomocysteinemia is a risk factor for early-onset venous thrombosis. It can be caused by genetic defects in methionine-homocysteine metabolism. The thermolabile variant of methylene-tetrahydrofolate reductase (MTHFR), C677C>T (Ala222Val), is one of the most common genetic condition, which has been associated with mild to moderate hyperhomocysteinemia, and carriers of this variant are at increased risk of an early-onset stroke-like episode. Two common non-synonymous variants, the C677T (Ala222Val) and A1298C (Glu429Ala), were described for the MTHFR gene and associated with a decreased enzymatic activity and an alteration of intracellular folate distribution.
DETAILS
Rely-AMP MTHFR is an in vitro Real Time polymerase chain reaction (RT- PCR) assay to detect mutations IN MTHFR. The assay allows for precise allelic discrimination of homozygous, heterozygous and wild type genotypes.Rely-AMP MTHFR provides all necessary reagents for reliable extraction andDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Qualitative
|
Extraction |
Magnetic Resin
|
Technology
|
Multiplex Real time PCR
|
Sample type |
Blood
|
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
|
Fluorophore
|
FAM - HEX
|
Processing |
Manual Automated
|
Time to result |
2 hours (after extraction)
|
PERFORMANCE
Diagnostic sensitivity: >94%
Diagnostic specificity: 100%
Analytical Specificity: no cross reazion
Analytical Sensitivity: 10 copies plasmid
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
PRICE |
Rely-AMP MTHFR extraction and detection kit |
MTHFR50 |
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Rely-AMP MTHFR detection kit |
MTHFR50D |
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