Rely-AMP MTHFR

 

 

Hyperhomocysteinemia is a risk factor for early-onset venous thrombosis. It can be caused by genetic defects in methionine-homocysteine metabolism. The thermolabile variant of methylene-tetrahydrofolate reductase (MTHFR), C677C>T (Ala222Val), is one of the most common genetic condition, which has been associated with mild to moderate hyperhomocysteinemia, and carriers of this variant are at increased risk of an early-onset stroke-like episode. Two common non-synonymous variants, the C677T (Ala222Val) and A1298C (Glu429Ala), were described for the MTHFR gene and associated with a decreased enzymatic activity and an alteration of intracellular folate distribution.

 

 

DETAILS

 

Rely-AMP MTHFR is an in vitro Real Time polymerase chain reaction (RT- PCR) assay to detect  mutations IN  MTHFR. The assay allows for precise allelic discrimination of homozygous, heterozygous and wild type genotypes.Rely-AMP MTHFR provides all necessary reagents for reliable extraction andDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

 

Quantitative/qualitative

 

Qualitative

 

Extraction

 

Magnetic Resin

 

 

Technology

 

 

Multiplex Real time PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Fluorophore

 

 

FAM - HEX

 

Processing

 

Manual

Automated

 

Time to result

 

2 hours (after extraction)

 

 

 

 

PERFORMANCE

 

Diagnostic sensitivity:  >94%

Diagnostic specificity: 100%

Analytical Specificity: no cross reazion

Analytical Sensitivity: 10 copies plasmid

 

 

ORDERING INFORMATION

 

 

PRODUCT

CAT.NO.

PRICE

 

Rely-AMP MTHFR extraction and detection kit

 

MTHFR50

 

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Rely-AMP  MTHFR  detection kit

 

MTHFR50D

 

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