Rely-AMP BCR-ABL P210

quantitative detection

 

Philadelphia chromosome is the result of a t(9;22) (q34;q11) reciprocal translocation and subsequent fusion of the c-ABL proto-oncogene on chromosome 9 with the BCR gene on chromosome 22.

Depending on the precise location of the fusion the molecular weight of the protein can range from 185 to 210 kDa. Two clinically important variants are the p190 and p210 isoforms. p190 is generally associated with  acute lymphoblastic leukemia (ALL), while p210 is generally associated with  chronic myeloid leukemia (CML) but can also be associated with ALL. Current guidelines from Europe Against Cancer (EAC) program and National Comprehensive Cancer Network (NCCN) recommend the use of quantitative real-time PCR to detect and quantify BCR-ABL expression before, during, and after treatment to assess the minimal residual disease (MRD) in patients with CML and ALL.

 

 

DETAILS

 

Rely-AMP BCR/ABL P210 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection p210 BCR-ABL mRNA. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP BCR/ABL P210 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

 

Quantitative/qualitative

 

 Quantitative

 

Extraction

 

 Guanidinium-phenol

 

Technology

 

 Real time PCR

 

Sample type

 

 Blood

Thermal cycler

 

 Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

Housekeeping gene

 

 ABL

 

Fluorophore

 

 FAM - HEX

 

Processing

 

 Manual

 

Time to result

 

 2 hours (after extraction)

 

  

PERFORMANCE

  

Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells 

Analytical Specificity: No cross-reactivity 

Diagnostic sensitivity: 96% 

Diagnostic specificity: 100%

 

 

Rely-AMP BCR-ABL P210

qualitative detection

 

 

DETAILS

 

Rely-AMP BCR/ABL  is an in vitro polymerase chain reaction assay for the direct detection BCR-ABL traslocation b3a2, b2a2, b2a3, b3a3. Rely-AMP BCR/ABL  provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

Quantitative/qualitative

 

 Qualitative

 

Extraction

 

 Guanidinium-phenol

 

Technology

 

 Nested RT-PCR

Sample type

 

 Blood

Thermal cycler

 

 Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

Control

 

 β-actine

 

Processing

 

 Manual- Agarose gel

 

Time to result

 

 3 hours (after extraction)

 

 

ORDERING INFORMATION

 

PRODUCT

CAT.NO.

PRICE

Rely-AMP BCR/ABL p210 extraction and detection kit

 

TTp210-50

 

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Rely-AMP BCR/ABL p210

detection kit

 

   TTp210-50D 

 

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Rely-AMP BCR/ABL p210 qualitative extraction and

detection kit

 

 

LTp210-50

 

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Rely-AMP BCR/ABL p210 qualitative detection kit

 

   LTp210-50D

 

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