Rely-AMP BCR-ABL P190

quantitative detection

 

 

 

Philadelphia chromosome is the result of a t(9;22) (q34;q11) reciprocal translocation and subsequent fusion of the c-ABL proto-oncogene on chromosome 9 with the BCR gene on chromosome 22.

Depending on the precise location of the fusion the molecular weight of the protein can range from 185 to 210 kDa. Two clinically important variants are the p190 and p210 isoforms. p190 is generally associated with acutelymphoblastic leukemia (ALL), while p210 is generally associated with chronic myeloid leukemia (CML) but can also be associated with ALL. Current guidelines from Europe Against Cancer (EAC) program and National Comprehensive Cancer Network (NCCN) recommend the use of quantitative real-time PCR to detect and quantify BCR-ABL expression before, during, and after treatment to assess the minimal residual disease (MRD) in patients with CML and ALL.

 

 

DETAILS

 

Rely-AMP BCR/ABL P190 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection p190 BCR-ABL mRNA. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP BCR/ABL P190 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

 

Quantitative/qualitative

 

Quantitative

 

Extraction

 

Guanidinium-phenol

 

Technology

 

Real time PCR

 

Sample type

 

Blood

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

Housekeeping gene

 

ABL

 

Fluorophore

 

FAM - HEX

Processing

 

Manual

Time to result

 

2 hours (after extraction)

 

 

PERFORMANCE

 

Analytical sensitivity-detection limit:  1 leukemic cell/105 normal cells

Analytical Specificity: No cross-reactivity

Diagnostic sensitivity:94,2%

Diagnostic specificity: 100%

 

 

 

 

Rely-AMP BCR-ABL P190 

qualitative detection

 

 

 

DETAILS

 

Rely-AMP BCR/ABL p190 qualitative detection is an in vitro polymerase chain reaction assay for the direct detection BCR-ABL p190 traslocation e1-a2, e1-a3. Rely-AMP BCR/ABL  p190 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

Quantitative/qualitative

 

Qualitative

 

Extraction

 

Guanidinium-phenol

 

Technology

 

Nested RT-PCR

 

Sample type

 

Blood

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

Control

 

β-actine

 

Processing

 

Manual- Agarose gel

Time to result

 

3 hours (after extraction)

 

 

ORDERING INFORMATION

 

 

PRODUCT

CAT.NO.

PRICE

Rely-AMP BCR/ABL p190 extraction and detection kit

 

TTp190-50

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Rely-AMP BCR/ABL p190 detection kit

TTp190-50D

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Rely-AMP BCR/ABL p190 qualitative extraction and detection kit

 

LTp190-50

 Contact us

Rely-AMP BCR/ABL p190 qualitative detection kit

 

LTp190-50D

 Contact us