Rely-AMP CBFB-MYH11

quantitative detection

 

 

CBFb-MYH11 fusion transcripts are expressed in acute myeloid leukemias (AML) and occur as a result of inversion 16 (p13q22) or t(16;16) chromosomal abnormalities, causing dysfunctional transcription and leukemogenesis.

 

DETAILS

 

Rely-AMP CBFB-MYH11 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection CBFB-MYH11 mRNA. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP CBFB-MYH11 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

Quantitative/qualitative

 

Quantitative

 

Extraction

 

Guanidinium-phenol

 

 

Technology

 

 

Real time PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Housekeeping gene

 

 

ABL

 

 

Fluorophore

 

 

FAM - HEX

 

Processing

 

Manual

 

 

Time to result

 

2 hours (after extraction)

 

 

 

 

 

 

PERFORMANCE

 

Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells

Analytical Specificity: No cross-reactivity

Diagnostic sensitivity:95%

Diagnostic specificity: 100%

 

 

Rely-AMP E2A-PBX1

qualitative detection

  

 

DETAILS

 

Rely-AMP CBFB-MYH11 qualitative detection is an in vitro polymerase chain reaction assay for the direct detection CBFB-MYH11 traslocation. Rely-AMP CBFB-MYH11 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

TECHNICAL SPECIFICATIONS

 

 

Quantitative/qualitative

 

Qualitative

 

Extraction

 

Guanidinium-phenol

 

 

Technology

 

 

Nested RT-PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Control

 

 

β-actine

 

Processing

 

Manual- Agarose gel

 

Time to result

 

3 hours (after extraction)

 

 

 

ORDERING INFORMATION

 

PRODOTTO

CAT.NO.

COSTO

Rely-AMP CBFB-MYH11 extraction and detection kit

 TTMYH11-50

Contact us

Rely-AMP CBFB-MYH11 detection kit

TTMYH11-50D

Contact us

Rely-AMP CBFB-MYH11 qualitative extraction and detection kit

LTMYH11-50

Contact us

Rely-AMP CBFB-MYH11 qualitative detection kit

 LTMYH11-50D

Contact us