Rely-AMP Factors II/V & MTHFR

(multiplex PCR kit)

                                                                                    

 

Venous Thromboembolism develops as the result of multiple interactions between non-genetic and genetic risk factors.Factor V Leiden and factor II G20210A mutations are two frequent genetic risk factors involved in venous thromboembolism. Both factor V Leiden and factor II mutation (G20210A) represent gain-of-function mutations: factor V Leiden causes resistance to activated protein C, and factor II mutation (G20210A) results in higher levels of plasma prothrombin.

Hyperhomocysteinemia is a risk factor for early-onset venous thrombosis. It can be caused by genetic defects in methionine-homocysteine metabolism. The thermolabile variant of methylene-tetrahydrofolate reductase (MTHFR), C677C>T, is one of the most common genetic condition, which has been associated with mild to moderate hyperhomocysteinemia, and carriers of this variant are at increased risk of an early-onset stroke-like episode. Two common non-synonymous variants, the C677T (Ala222Val) and A1298C (Glu429Ala), were described for the MTHFR gene and associated with a decreased enzymatic activity and an alteration of intracellular folate distribution.

 

 

DETAILS

 

Rely-AMP FACTORS II/V & MTHFR is an in vitro Real Time polymerase chain reaction (RT- PCR) assay to detect  mutations factor  II, V leiden and MTHFR. 

The assay allows for precise allelic discrimination of homozygous, heterozygous and wild type genotypes.

Rely-AMP FACTORS II/V & MTHFR provides all necessary reagents for reliable extraction andDNA detection.

 

TECHNICAL SPECIFICATIONS

 

 

Quantitative/qualitative

 

Qualitative

 

Extraction

 

Magnetic Resin

 

 

Technology

 

 

Multiplex Real time PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Fluorophore

 

 

FAM- TEXAX RED-HEX

 

Processing

 

Manual

Automated

 

Time to result

 

2 hours (after extraction)

 

 

 

PERFORMANCE

 

Diagnostic sensitivity:  > 95%

Diagnostic specificity: 100%

Analytical Specificity: no cross reazione

Analytical sensitivity: 10 copies plasmid

 

 

ORDERING INFORMATION

 

PRODUCT

CAT.NO.

PRICE

 

Rely-AMP FACTORS II/V & MTHFR extraction and detection kit

 

CFM50

 

Contact us

 

Rely-AMP FACTORS II/V & MTHFR detection kit

 

CFM50D

 

Contact us