Rely-AMP FACTORS II/V
(multiplex PCR KIT)
Venous Thromboembolism develops as the result of multiple interactions between non-genetic and genetic risk factors.Factor V Leiden and factor II G20210A mutations are two frequent genetic risk factors involved in venous thromboembolism.
Individuals with factor V Leiden have a mutation at one of the arginine cleavage sites in factor V, such that factor V resists degradation by activated protein C. Persons who are heterozygous or homozygous for this disorder are 8 times and 80 times increased risk, respectively, for VTE.
The factor II mutation (G20210A) is associated with increased plasma prothrombin levels and is present in 1% to 2% of the general population. Heterozygote carriers of the prothrombin G20210A mutation have an estimated 3 to 8-fold increased risk for venous thrombosis.
DETAILS
Rely-AMP FACTORS II/V is an in vitro Real Time polymerase chain reaction (RT- PCR) assay to detect mutations factor II and V leiden. The assay allows for precise allelic discrimination of homozygous, heterozygous and wild type genotypes. Rely-AMP FACTORS II/V provides all necessary reagents for reliable extraction and DNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Qualitative
|
Extraction |
Magnetic Resin
|
Technology
|
Multiplex Real time PCR
|
Sample type |
Blood
|
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
|
Fluorophore
|
FAM - TEXAX RED
|
Processing |
Manual Automated
|
Time to result |
2 hours (after extraction)
|
PERFORMANCE
Diagnostic sensitivity: >94%
Diagnostic specificity: 100%
Analytical Specificity: no cross reazione
Analytical Sensitivity: 10 copies plasmid
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
PRICE |
Rely-AMP FACTORS II/V extraction and detection kit |
II/V50 |
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Rely-AMP FACTORS II/V detection kit |
II/V50D |
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