Rely-AMP FACTORS II/V

(multiplex PCR KIT)

 

Venous Thromboembolism develops as the result of multiple interactions between non-genetic and genetic risk factors.Factor V Leiden and factor II G20210A mutations are two frequent genetic risk factors involved in venous thromboembolism.

Individuals with factor V Leiden have a mutation at one of the arginine cleavage sites in factor V, such that factor V resists degradation by activated protein C. Persons who are heterozygous or homozygous for this disorder are 8 times and 80 times increased risk, respectively, for VTE.

The factor II mutation (G20210A) is associated with increased plasma prothrombin levels and is present in 1% to 2% of the general population. Heterozygote carriers of the prothrombin G20210A mutation have an estimated 3 to 8-fold increased risk for venous thrombosis.

 

 

DETAILS

 

Rely-AMP FACTORS II/V  is an in vitro Real Time polymerase chain reaction (RT- PCR) assay to detect mutations factor  II and V leiden. The assay allows for precise allelic discrimination of homozygous, heterozygous and wild type genotypes. Rely-AMP FACTORS II/V  provides all necessary reagents for reliable extraction and DNA detection.

 

 

 

TECHNICAL SPECIFICATIONS

 

 

 

Quantitative/qualitative

 

Qualitative

 

Extraction

 

Magnetic Resin

 

 

Technology

 

 

Multiplex Real time PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Fluorophore

 

 

FAM - TEXAX RED

 

Processing

 

Manual

Automated

 

Time to result

 

2 hours (after extraction)

 

 

 

 

PERFORMANCE

 

 

Diagnostic sensitivity:  >94%

Diagnostic specificity: 100%

Analytical Specificity: no cross reazione

Analytical Sensitivity: 10 copies plasmid

 

 

 

ORDERING INFORMATION

 

 

PRODUCT

CAT.NO.

PRICE

 

Rely-AMP FACTORS II/V extraction and detection kit

 

II/V50

 

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Rely-AMP  FACTORS II/V  detection kit

 

II/V50D

 

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