RELY-AMP SIL-TAL1

quantitative detection

The TAL-1 gene, also known as SCL or TCL5 located on 1p32, encodes a basic helix loop helix protein Tal-1 and is essential for the earliest stages of hematopoietic stem cell development and differentiation.

In about 20% of T-cell acute lymphoblastic leukemias (T-ALL), this gene is disrupted in its 5' portion by a site-specific 100-kb deletion and is fused with the 5' part of the SIL gene, to form SIL-TAL-1 chimeric gene.

DETAILS

Rely-AMP SIL_TAL1 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection SIL_TAL1 mRNA. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP SIL_TAL provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

TECHNICAL SPECIFICATIONS

PERFORMANCE

Analytical sensitivity-detection limit: 1 leukemic cell/10⁵ normal cells 

Analytical Specificity: No cross-reactivity 

Diagnostic sensitivity:95% 

Diagnostic specificity: 100%

RELY-AMP SIL-TAL1

qualitative detection

Rely-AMP SIL-TAL1  is an in vitro polymerase chain reaction assay for the direct detection SIL-TAL1 traslocation. Rely-AMP SIL-TAL1  provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

TECHNICAL SPECIFICATIONS

ORDERING INFORMATION