RELY-AMP SIL-TAL1
quantitative detection
The TAL-1 gene, also known as SCL or TCL5 located on 1p32, encodes a basic helix loop helix protein Tal-1 and is essential for the earliest stages of hematopoietic stem cell development and differentiation.
In about 20% of T-cell acute lymphoblastic leukemias (T-ALL), this gene is disrupted in its 5' portion by a site-specific 100-kb deletion and is fused with the 5' part of the SIL gene, to form SIL-TAL-1 chimeric gene.
DETAILS
Rely-AMP SIL_TAL1 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection SIL_TAL1 mRNA. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP SIL_TAL provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Quantitative
|
Extraction |
Guanidinium-phenol
|
Technology
|
Real time PCR
|
Sample type |
Blood
|
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
|
Housekeeping gene
|
ABL
|
Fluorophore
|
FAM - HEX
|
Processing |
Manual
|
Time to result |
2 hours (after extraction)
|
PERFORMANCE
Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells
Analytical Specificity: No cross-reactivity
Diagnostic sensitivity:95%
Diagnostic specificity: 100%
RELY-AMP SIL-TAL1
qualitative detection
Rely-AMP SIL-TAL1 is an in vitro polymerase chain reaction assay for the direct detection SIL-TAL1 traslocation. Rely-AMP SIL-TAL1 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Qualitative
|
Extraction |
Guanidinium-phenol
|
Technology
|
Nested RT-PCR
|
Sample type |
Blood
|
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
|
Control
|
β-actine
|
Processing |
Manual- Agarose gel
|
Time to result |
3 hours (after extraction) |
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
COSTO |
Rely-AMP SIL-TAL1 extraction and detection kit |
TTTAL1-50 |
Contact us |
Rely-AMP SIL-TAL1 detection kit |
TTTAL1-50D
|
Contact us |
Rely-AMP SIL-TAL1 qualitative extraction and detection kit |
LTTAL1-50 |
Contact us |
Rely-AMP SIL-TAL1 qualitative detection kit |
LTTAL1-50D |
Contact us |