Rely-AMP MLL-AF4

quantitative detection

 

 

The chromosomal translocation occurring between the band 21 of the long arm of chromosome 4 and band 23 of the long arm of chromosome 11 t(4;11)(q21;q23) and leading to the generation of the fusion gene MLL-AF4 is one of the most recurrent chromosomal aberrations observed in acute lymphoblastic leukemia (ALL). However, a diagnosis of t(4;11)(q21;q23)/MLL-AF4 positive ALL in adult patients is a rare event, considering the relative low incidence of ALL in adult population.

The incidence of t(4;11)(q21;q23)/MLL-AF4 positive ALL has a characteristic bimodal age distribution with a major peak in early infancy, occurring in over 50% ALL cases in infants aged less than 6 months, in 10–20% of older infants, in about 2% of children, and in almost 10% of adults.

 

 

DETAILS

 

Rely-AMP MLL-AF4 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection MLL-AF4 fusion transcript. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP MLL-AF4 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

Quantitative/qualitative

 

Quantitative

 

Extraction

 

Guanidinium-phenol

 

 

Technology

 

 

Real time PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Housekeeping gene

 

 

ABL

 

 

Fluorophore

 

 

FAM - HEX

 

Processing

 

Manual

 

 

Time to result

 

2 hours (after extraction)

 

 

 

PERFORMANCE

 

Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells 

Analytical Specificity: No cross-reactivity 

Diagnostic sensitivity:95,3% 

Diagnostic specificity: 100%

 

 

 

Rely-AMP MLL-AF4

 

qualitative detection

 

 

Rely-AMP MLL-AF4 qualitative detection is an in vitro polymerase chain reaction assay for the direct detection MLL-AF4 traslocation e8-e7, e8-e4, e9-e5, e9-e4, e10-e6, e10-e5, e10-e4, e11-e6, e11-e5, e11-e4. Rely-AMP T MLL-AF4 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

Quantitative/qualitative

 

Qualitative

 

Extraction

 

Guanidinium-phenol

 

 

Technology

 

 

Nested RT-PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Control

 

 

β-actine

 

Processing

 

Manual- Agarose gel

 

Time to result

 

3 hours (after extraction)

 

 

ORDERING INFORMATION

 

PRODOTTO

CAT.NO.

COSTO

Rely-AMP MLL-AF4 extraction and detection kit

TTAF4-50

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Rely-AMP MLL-AF4 detection kit

TTAF4-50D

Contact us

Rely-AMP MLL-AF4 qualitative extraction and detection kit

LTAF4-50

Contact us

Rely-AMP MLL-AF4 qualitative detection kit

LTAF4-50D

Contact us