Rely-AMP Factors II/V & MTHFR

(multiplex PCR kit)

Venous Thromboembolism develops as the result of multiple interactions between non-genetic and genetic risk factors.Factor V Leiden and factor II G20210A mutations are two frequent genetic risk factors involved in venous thromboembolism. Both factor V Leiden and factor II mutation (G20210A) represent gain-of-function mutations: factor V Leiden causes resistance to activated protein C, and factor II mutation (G20210A) results in higher levels of plasma prothrombin.

Hyperhomocysteinemia is a risk factor for early-onset venous thrombosis. It can be caused by genetic defects in methionine-homocysteine metabolism. The thermolabile variant of methylene-tetrahydrofolate reductase (MTHFR), C677C>T, is one of the most common genetic condition, which has been associated with mild to moderate hyperhomocysteinemia, and carriers of this variant are at increased risk of an early-onset stroke-like episode. Two common non-synonymous variants, the C677T (Ala222Val) and A1298C (Glu429Ala), were described for the MTHFR gene and associated with a decreased enzymatic activity and an alteration of intracellular folate distribution.

DETAILS

Rely-AMP FACTORS II/V & MTHFR is an in vitro Real Time polymerase chain reaction (RT- PCR) assay to detect  mutations factor  II, V leiden and MTHFR. 

The assay allows for precise allelic discrimination of homozygous, heterozygous and wild type genotypes.

Rely-AMP FACTORS II/V & MTHFR provides all necessary reagents for reliable extraction andDNA detection.

TECHNICAL SPECIFICATIONS

PERFORMANCE

Diagnostic sensitivity:  > 95%

Diagnostic specificity: 100%

Analytical Specificity: no cross reazione

Analytical sensitivity: 10 copies plasmid

ORDERING INFORMATION