RELY-AMP SIL-TAL1

quantitative detection

 

 

 

The TAL-1 gene, also known as SCL or TCL5 located on 1p32, encodes a basic helix loop helix protein Tal-1 and is essential for the earliest stages of hematopoietic stem cell development and differentiation.

In about 20% of T-cell acute lymphoblastic leukemias (T-ALL), this gene is disrupted in its 5' portion by a site-specific 100-kb deletion and is fused with the 5' part of the SIL gene, to form SIL-TAL-1 chimeric gene.

 

 

DETAILS

 

Rely-AMP SIL_TAL1 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection SIL_TAL1 mRNA. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP SIL_TAL provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

 

TECHNICAL SPECIFICATIONS

 

 

 

Quantitative/qualitative

 

Quantitative

 

Extraction

 

Guanidinium-phenol

 

 

Technology

 

 

Real time PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Housekeeping gene

 

 

ABL

 

 

Fluorophore

 

 

FAM - HEX

 

Processing

 

Manual

 

 

Time to result

 

2 hours (after extraction)

 

 

 

PERFORMANCE

 

Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells 

Analytical Specificity: No cross-reactivity 

Diagnostic sensitivity:95% 

Diagnostic specificity: 100%

 

 

 

 

RELY-AMP SIL-TAL1

qualitative detection

 

 

Rely-AMP SIL-TAL1  is an in vitro polymerase chain reaction assay for the direct detection SIL-TAL1 traslocation. Rely-AMP SIL-TAL1  provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

 

TECHNICAL SPECIFICATIONS

 

 

 

Quantitative/qualitative

 

Qualitative

 

Extraction

 

Guanidinium-phenol

 

 

Technology

 

 

Nested RT-PCR

 

Sample type

 

Blood

 

Thermal cycler

 

optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers

 

 

Control

 

 

β-actine

 

Processing

 

Manual- Agarose gel

 

Time to result

 

3 hours (after extraction)

 

 

ORDERING INFORMATION

 

PRODUCT

CAT.NO.

COSTO

Rely-AMP SIL-TAL1 extraction and detection kit

TTTAL1-50

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Rely-AMP SIL-TAL1 detection kit

 

TTTAL1-50D

 

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Rely-AMP SIL-TAL1 qualitative extraction and detection kit

LTTAL1-50

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Rely-AMP SIL-TAL1 qualitative detection kit

LTTAL1-50D

Contact us