Rely-AMP HPV High Risk
Human papillomavirus (HPV) is a DNA virus, that is capable of establishing productive infections only in keratinocytes of the skin or mucous membranes. HPV infections are believed to cause 96% of cervical cancer. More than 120 genotypes of HPV have been identified, of these, 14 genotypes have been classified as “high-risk HPV” (HR-HPV) because they can lead to cervical cancer.
High Risk HPV |
Frequency Europe (%) |
16 |
58,1 |
18 |
15,7 |
31 |
4,0 |
33 |
4,4 |
35 |
1,6 |
39 |
0,2 |
45 |
2,9 |
51 |
0,2 |
52 |
0,6 |
56 |
1,2 |
58 |
1,0 |
59 |
0,2 |
66 |
0,2 |
68 |
0,2 |
DETAILS
Rely-AMP HPV is an in vitro polymerase chain reaction (PCR) assay for the direct, qualitative detection of 14 HPV High Risk from clinical specimens. Rely-AMP HPV provides all necessary reagents for reliable detection of all High Risk HPV.
The kit is designed for the detection of the High Risk HPV DNA, based on the amplification of the specific sequence of the L1 region of Papilloma virus, by Real time PCR multiplex. The system has an internal control, based on amplification of Human RNase. Furthermore Rely-AMP HPV allows the genotyping of the HPV 16 and HPV 18.
TECHNICAL SPECIFICATION
Quantitative/qualitative |
Qualitative |
Extraction |
Magnetic Resin |
Technology |
Multiplex Real time PCR |
Sample type |
Cervical swabs in Medium PreservCyt |
Thermal cycler |
Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers |
Internal control |
RNasi gene |
Positive control |
HPV plasmid DNA |
Amplified region |
L1 |
Fluorophore |
FAM - HEX - CY5 |
Processing |
Manual Automated |
Time to result |
2 hours (after extraction) |
PERFORMANCE
Diagnostic sensitivity: 90%
Diagnostic specificity: 98%
Analytical Specificity: no cross reaction
Analytical sensitivity:
High Risk HPV |
Copies/ml |
16 |
300 |
18 |
500 |
31 |
500 |
33 |
500 |
35 |
500 |
39 |
500 |
45 |
300 |
51 |
300 |
52 |
1500 |
56 |
600 |
58 |
600 |
59 |
600 |
66 |
1500 |
68 |
1500 |
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
PRICE |
Rely-AMP HPV extraction and detection kit |
HPV HR50 |
Contact us |
Rely-AMP HPV detection kit |
HPV HR50D |
Contact us |