TEL-AML1
quantitative detection
The TEL(ETV6) /AML1(CBFA2,RUNX1) is a gene fusion resulting from a t(12;21)(p13;q22) chromosomal translocation. Although it occurs in only about 3% of adult acute lymphoblastic leukemia (ALL) cases, it is the most common genetic rearrangement in B-lineage pediatric ALL (frequency ~25%). The breakpoint at TEL occurs invariably in intron 5 and three translocation breakpoints in AML1 have been described, although two are more common. The TEL moiety of the chimeric protein converts AML1 from an activator to a transcriptional repressor. However, alternative mechanisms of TEL-AML1 activity have also been suggested like sequestration of transcriptional cofactors to the cytoplasm or dimerization with wild-type protein.
DETAILS
Rely-AMP TEL/AML1 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection TEL/AML1 fusion transcript. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP TEL/AML1 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Quantitative |
Extraction |
Guanidinium-phenol |
Technology |
Real time PCR |
Sample type |
Blood |
Thermal cycler |
Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers |
Housekeeping gene |
ABL |
Fluorophore |
FAM - HEX |
Processing |
Manual |
Time to result |
2 hours (after extraction) |
PERFORMANCE
Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells
Analytical Specificity: No cross-reactivity
Diagnostic sensitivity:94,6%
Diagnostic specificity: 100%
TEL-AML1
qualitative detection
DETAILS
Rely-AMP TEL/AML1 qualitative detection is an in vitro polymerase chain reaction assay for the direct detection TEL/AML1 traslocation. Rely-AMP TEL/AML1 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Qualitative |
Extraction |
Guanidinium-phenol |
Technology |
Nested RT-PCR |
Sample type |
Blood |
Thermal cycler |
Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers |
Control |
β-actine |
Processing |
Manual- Agarose gel |
Time to result |
3 hours (after extraction) |
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
PRICE |
Rely-AMP TEL-AML1 extraction and detection kit |
TTAML1-50 |
Contact us |
Rely-AMP TEL-AML1 detection kit |
TTAML1-50D |
Contact us |
Rely-AMP TEL-AML1 qualitative extraction and detection kit |
LTAML1-50D |
Contact us |
Rely-AMP TEL-AML1 qualitative detection kit |
LTAML1-50D |
Contact us |