Rely-AMP CBFB-MYH11
quantitative detection
CBFb-MYH11 fusion transcripts are expressed in acute myeloid leukemias (AML) and occur as a result of inversion 16 (p13q22) or t(16;16) chromosomal abnormalities, causing dysfunctional transcription and leukemogenesis.
DETAILS
Rely-AMP CBFB-MYH11 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection CBFB-MYH11 mRNA. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP CBFB-MYH11 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
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Quantitative/qualitative |
Quantitative
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Extraction |
Guanidinium-phenol
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Technology
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Real time PCR
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Sample type |
Blood
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Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
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Housekeeping gene
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ABL
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Fluorophore
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FAM - HEX
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Processing |
Manual
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Time to result |
2 hours (after extraction)
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PERFORMANCE
Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells
Analytical Specificity: No cross-reactivity
Diagnostic sensitivity:95%
Diagnostic specificity: 100%
Rely-AMP E2A-PBX1
qualitative detection
DETAILS
Rely-AMP CBFB-MYH11 qualitative detection is an in vitro polymerase chain reaction assay for the direct detection CBFB-MYH11 traslocation. Rely-AMP CBFB-MYH11 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
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Quantitative/qualitative |
Qualitative
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Extraction |
Guanidinium-phenol
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Technology
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Nested RT-PCR
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Sample type |
Blood
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Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
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Control
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β-actine
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Processing |
Manual- Agarose gel
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Time to result |
3 hours (after extraction)
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ORDERING INFORMATION
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PRODOTTO |
CAT.NO. |
COSTO |
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Rely-AMP CBFB-MYH11 extraction and detection kit |
TTMYH11-50 |
Contact us |
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Rely-AMP CBFB-MYH11 detection kit |
TTMYH11-50D |
Contact us |
|
Rely-AMP CBFB-MYH11 qualitative extraction and detection kit |
LTMYH11-50 |
Contact us |
|
Rely-AMP CBFB-MYH11 qualitative detection kit |
LTMYH11-50D |
Contact us |