Rely-AMP BCR-ABL P210
quantitative detection
Philadelphia chromosome is the result of a t(9;22) (q34;q11) reciprocal translocation and subsequent fusion of the c-ABL proto-oncogene on chromosome 9 with the BCR gene on chromosome 22.
Depending on the precise location of the fusion the molecular weight of the protein can range from 185 to 210 kDa. Two clinically important variants are the p190 and p210 isoforms. p190 is generally associated with acute lymphoblastic leukemia (ALL), while p210 is generally associated with chronic myeloid leukemia (CML) but can also be associated with ALL. Current guidelines from Europe Against Cancer (EAC) program and National Comprehensive Cancer Network (NCCN) recommend the use of quantitative real-time PCR to detect and quantify BCR-ABL expression before, during, and after treatment to assess the minimal residual disease (MRD) in patients with CML and ALL.
DETAILS
Rely-AMP BCR/ABL P210 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection p210 BCR-ABL mRNA. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP BCR/ABL P210 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Quantitative |
Extraction |
Guanidinium-phenol |
Technology |
Real time PCR |
Sample type |
Blood |
Thermal cycler |
Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers |
Housekeeping gene |
ABL |
Fluorophore |
FAM - HEX |
Processing |
Manual |
Time to result |
2 hours (after extraction) |
PERFORMANCE
Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells
Analytical Specificity: No cross-reactivity
Diagnostic sensitivity: 96%
Diagnostic specificity: 100%
Rely-AMP BCR-ABL P210
qualitative detection
DETAILS
Rely-AMP BCR/ABL is an in vitro polymerase chain reaction assay for the direct detection BCR-ABL traslocation b3a2, b2a2, b2a3, b3a3. Rely-AMP BCR/ABL provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Qualitative |
Extraction |
Guanidinium-phenol |
Technology |
Nested RT-PCR |
Sample type |
Blood |
Thermal cycler |
Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers |
Control |
β-actine |
Processing |
Manual- Agarose gel |
Time to result |
3 hours (after extraction) |
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
PRICE |
Rely-AMP BCR/ABL p210 extraction and detection kit |
TTp210-50 |
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Rely-AMP BCR/ABL p210 detection kit |
TTp210-50D |
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Rely-AMP BCR/ABL p210 qualitative extraction and detection kit |
LTp210-50 |
Contact us |
Rely-AMP BCR/ABL p210 qualitative detection kit |
LTp210-50D |
Contact us |