AML1-ETO

quantitative detection

Translocation t(8;21)(q22;q22) fuses the 5’-portion of the AML1 gene on chromosome 21 to the almost complete open reading frame of the ETO gene on chromosome 8 generating an AML1-ETO fusion transcript. The breakpoints of AML1 are located between exon 5 and 6 and upstream of exon 2 regarding ETO. The translocation t(8;21)(q22;q22) is one of the most common structural chromosomal aberrations in patients with acute myeloid leukemia (AML).

The quantitative t(8;21) assay can be used not only to diagnose AML, but also to serially monitor patients for MRD (minimal residual disease), evaluate the effectiveness of treatment, and predict early relapse.

DETAILS

Rely-AMP AML1-ETO is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection AML1-ETO fusion transcript. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP AML1-ETO provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

TECHNICAL SPECIFICATIONS

PERFORMANCE

Analytical sensitivity-detection limit: 1 leukemic cell/10⁵ normal cells

Analytical Specificity: No cross-reactivity

Diagnostic sensitivity:94,8%

Diagnostic specificity: 100%

AML1-ETO

 qualitative detection

DETAILS

Rely-AMP AML1-ETO qualitative detection is an in vitro polymerase chain reaction assay for the direct detection AML1-ETO traslocation M1, M2, M4. Rely-AMP AML1-ETO provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

TECHNICAL SPECIFICATIONS

ORDERING INFORMATION