Rely-AMP MYCOPLASMAS
(multiplex pcr kit)
M. hominis M genitalium and U. urealyticum colonise the genitourinary tract of humans. These organisms can cause urethritis and contribute to vaginitis in women. They have been associated with sexually transmitted diseases and with chronic infections in people with weakened immune systems. Genitourinary infections associated with Mycoplasma hominis, Ureaplasma urealyticum Mycoplasma genitalium include: nongonococcal urethritis, pelvic inflammatory disease, infertility, pyelonephritis and urinary calculi. Many studies show that mycoplasma leads to male infertility through decreasing sperm motility and invasion of the testes.
DETAILS
Rely-AMP MYCOPLASMAS is an in vitro polymerase chain reaction (PCR) assay for the direct, qualitative detection of Ureaplasma urealyticum, Mycoplasma hominis e Mycoplasma genitalium from urogenital specimens. Rely-AMP MYCOPLASMAS provides all necessary reagents for reliable Ureaplasma urealyticum, Mycoplasma hominis e Mycoplasma genitalium extraction andDNA detection.
The kit is designed for the detection of the Ureaplasma urealyticum, Mycoplasma hominis e Mycoplasma genitalium DNA based on the amplification of the specific sequence of the U. urealyticum urease gene, M. hominis GAPDH gene, and M genitalium repetitive sequence element mgp-r2 and on the amplification of the sequence of the gene for RNasi by means of the Real time PCR.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Qualitative
|
Extraction |
Magnetic Resin
|
Technology
|
Multiplex Real time PCR
|
Sample type |
Urine, cervical swabs, urethral swabs, sperm,
|
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
|
Internal control
|
RNasi gene
|
Positive control |
Ureaplasma urealyticum, Mycoplasma hominis e Mycoplasma genitalium plasmid DNA
|
Amplified region |
U. urealyticum:urease gene, M. hominis:GAPDH gene, M genitalium: repetitive sequence element mgp-r2
|
Fluorophore
|
FAM - CY5 - HEX
|
Processing |
Manual Automated
|
Time to result |
2 hours (after extraction)
|
PERFORMANCE
Diagnostic sensitivity: 94,2%
Diagnostic specificity: 99,9%
Analytical Specificity: no cross reazione
Analytical sensitivity: 10 copies plasmid
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
COSTO |
Rely-AMP MYCOPLASMAS extraction and detection kit |
MY50 |
Contact us |
Rely-AMP MYCOPLASMAS detection kit |
MY50D |
Contact us |