Rely-AMP PML-RARA
quantitative detection
Acute Promyelocytic leukemia (APL or AML-M3) is a subtype of acute myeloid leukemia with distinct clinical and histopathologic features.Genetically, APL cells contain a characteristic translocation fusion gene comprised of the downstream sequences of the retinoic acid receptor alpha gene (RARA) fused to the promoter region and upstream sequences of the promyelocytic leukemia gene (PML). The fusion gene is designated PML/RARA and is the consequence of the t(15;17)(q22;q21) translocation. Depending on the location of breakpoints within the PML site, intron 6, exon 6 and intron 3, the respective PML-RARa transcript subtypes referred to as long (L or bcr1), variant (V or bcr2) and short (S or bcr3), may be formed. Recent studies have indicated that sensitive monitoring is important because the majority of patients who remain PCR-positive, or become PCR-positive again following treatment, will relapse and likely benefit from early intervention for residual/recurrent disease.
DETAILS
Rely-AMP PML-RARA is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection PML-RARA fusion transcript. Housekeeping gene ABL is a reference for relative quantitation.Rely-AMP PML-RARA provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Quantitative |
Extraction |
Guanidinium-phenol |
Technology |
Real time PCR |
Sample type |
Blood |
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers |
Housekeeping gene |
ABL |
Fluorophore |
FAM - HEX |
Processing |
Manual |
Time to result |
2 hours (after extraction) |
PERFORMANCE
Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells
Analytical Specificity: No cross-reactivity
Diagnostic sensitivity:95%
Diagnostic specificity: 100%
Rely-AMP PML-RARA
qualitative detection
Rely-AMP PML-RARA qualitative detection is an in vitro polymerase chain reaction assay for the direct detection PML-RARA traslocation bcr1, bcr2, bcr3. Rely-AMP PML-RARA provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Qualitative |
Extraction |
Guanidinium-phenol |
Technology |
Nested RT-PCR |
Sample type |
Blood |
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers |
Control |
β-actine |
Processing |
Manual- Agarose gel |
Time to result |
3 hours (after extraction) |
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
PRICE |
Rely-AMP PML-RARA extraction and detection kit |
TTRARA-50 |
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Rely-AMP PML-RARA detection kit |
TTRARA-50D |
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Rely-AMP PML-RARA qualitative extraction and detection kit |
LTRARA-50 |
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Rely-AMP PML-RARA qualitative detection kit |
|
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