Rely-AMP HPV 16-18
Human papillomavirus (HPV) is a DNA virus, that is capable of establishing productive infections only in keratinocytes of the skin or mucous membranes. HPV infections are believed to cause 96% of cervical cancer. More than 120 genotypes of HPV have been identified, of these, 14 genotypes have been classified as “high-risk HPV” (HR-HPV) because they can lead to cervical cancer. Type 16 is the cause of approximately 50% of cervical cancers worldwide, and types 16 and 18 together account for about 70% of cervical cancers.
High Risk HPV |
Frequency Europe (%) |
16 |
58,1 |
18 |
15,7 |
DETAILS
Rely-AMP HPV is an in vitro polymerase chain reaction (PCR) assay for the direct, qualitative detection of HPV High Risk 16 and 18, from clinical specimens.
The kit is designed for the detection of the High Risk HPV DNA, based on the amplification of the specific sequence of the L1 region of Papilloma virus, by Real time PCR multiplex. The system has an internal control, based on amplification of Human RNase.
TECHNICAL SPECIFICATION
Quantitative/qualitative |
Qualitative |
Extraction |
Magnetic resin |
Technology |
Multiplex Real time PCR |
Sample type |
PreservCyt medium |
Thermal cycler |
Optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers |
Internal control |
RNasi gene |
Positive control |
HPV plasmid DNA |
Fluorophore |
FAM - HEX - CY5 |
Processing |
Manual and Automated |
Time to result |
2 hours (after extraction) |
PERFORMANCE
Diagnostic sensitivity: 98%
Diagnostic specificity: 100%
Analytical specificity: no cross-reaction
Analytical sensitivity:
High Risk HPV |
Copie/ml |
16 |
300 |
18 |
500 |
ORDERING INFORMATION
PRODUCT |
CAT.NO. |
PRICE |
Rely-AMP HPV extraction and detection kit |
16-18HPV 50 |
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Rely-AMP HPV detection kit |
16-18HPV 50D |
Contact us |