Rely-AMP MLL-AF4

quantitative detection

The chromosomal translocation occurring between the band 21 of the long arm of chromosome 4 and band 23 of the long arm of chromosome 11 t(4;11)(q21;q23) and leading to the generation of the fusion gene MLL-AF4 is one of the most recurrent chromosomal aberrations observed in acute lymphoblastic leukemia (ALL). However, a diagnosis of t(4;11)(q21;q23)/MLL-AF4 positive ALL in adult patients is a rare event, considering the relative low incidence of ALL in adult population.

The incidence of t(4;11)(q21;q23)/MLL-AF4 positive ALL has a characteristic bimodal age distribution with a major peak in early infancy, occurring in over 50% ALL cases in infants aged less than 6 months, in 10–20% of older infants, in about 2% of children, and in almost 10% of adults.

DETAILS

Rely-AMP MLL-AF4 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection MLL-AF4 fusion transcript. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP MLL-AF4 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

TECHNICAL SPECIFICATIONS

PERFORMANCE

Analytical sensitivity-detection limit: 1 leukemic cell/10⁵ normal cells 

Analytical Specificity: No cross-reactivity 

Diagnostic sensitivity:95,3% 

Diagnostic specificity: 100%

Rely-AMP MLL-AF4

qualitative detection

Rely-AMP MLL-AF4 qualitative detection is an in vitro polymerase chain reaction assay for the direct detection MLL-AF4 traslocation e8-e7, e8-e4, e9-e5, e9-e4, e10-e6, e10-e5, e10-e4, e11-e6, e11-e5, e11-e4. Rely-AMP T MLL-AF4 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.

TECHNICAL SPECIFICATIONS

ORDERING INFORMATION