Rely-AMP E2A-PBX1
quantitative detection
The leukemogenic fusion gene transcript E2A-PBX1 results from fusion of the E2A and PBX1 (formerly prl) genes, through the t(1;19)(q23;p13). The t(1;19) is detected in about 5–6% of childhood acute lymphoblastic leukemia and in about 3% of adult acute lymphoblastic leukemia. In the majority of cases the E2A-PBX1 fusion transcript shows a constant junction site of exon 13 (nucleotide 1518) of E2A26 to the second exon (nucleotide 388) of PBX.
A variant fusion transcript is described in about 5–10% of t(1;19)-positive acute lymphoblastic leukemia. It is characterized by a stretch of 27 nucleotides inserted at the usual junction point between nucleotides 1518 and 388 of the E2A and PBX1 genes, respectively.
DETAILS
Rely-AMP E2A-PBX1 is an in vitro Real Time polymerase chain reaction assay for the direct, quantitative detection E2A-PBX1 fusion transcript. Housekeeping gene ABL is a reference for relative quantitation. Rely-AMP E2A-PBX1 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Quantitative
|
Extraction |
Guanidinium-phenol
|
Technology
|
Real time PCR
|
Sample type |
Blood
|
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
|
Housekeeping gene
|
ABL
|
Fluorophore
|
FAM - HEX
|
Processing |
Manual
|
Time to result |
2 hours (after extraction)
|
PERFORMANCE
Analytical sensitivity-detection limit: 1 leukemic cell/105 normal cells
Analytical Specificity: No cross-reactivity
Diagnostic sensitivity:95%
Diagnostic specificity: 100%
Rely-AMP E2A-PBX1
qualitative detection
DETAILS
Rely-AMP E2A-PBX1 qualitative detection is an in vitro polymerase chain reaction assay for the direct detection E2A-PBX1 traslocation E2A-A_PBX1-B, E2A-C_ PBX1-E3´, E2A-C_ PBX1-D. Rely-AMP E2A-PBX1 provides all necessary reagents for RNA extraction, reverse transcription and cDNA detection.
TECHNICAL SPECIFICATIONS
Quantitative/qualitative |
Qualitative
|
Extraction |
Guanidinium-phenol
|
Technology
|
Nested RT-PCR
|
Sample type |
Blood
|
Thermal cycler |
optimized with CFX96 (Bio-Rad) but designed for use with real-time devices from various manufacturers
|
Control
|
β-actine
|
Processing |
Manual- Agarose gel
|
Time to result |
3 hours (after extraction)
|
ORDERING INFORMATION
PRODOTTO |
CAT.NO. |
COSTO |
Rely-AMP E2A-PBX1 extraction and detection kit |
TTPBX1-50 |
Contact us |
Rely-AMP E2A-PBX1 detection kit |
TTPBX1-50D |
Contact us |
Rely-AMP E2A-PBX1 qualitative extraction and detection kit |
LTPBX1-50 |
Contact us |
Rely-AMP E2A-PBX1 qualitative detection kit |
LTPBX1-50D |
Contact us |