{"id":78,"date":"2021-11-30T16:33:58","date_gmt":"2021-11-30T15:33:58","guid":{"rendered":"http:\/\/127.0.0.1\/?page_id=78"},"modified":"2026-01-09T16:03:11","modified_gmt":"2026-01-09T15:03:11","slug":"microbscan","status":"publish","type":"page","link":"https:\/\/nurex.it\/en\/microbscan\/","title":{"rendered":"MicrobScan"},"content":{"rendered":"
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MicrobScan Diagnostic system<\/strong><\/p>\n

The high efficiency and the main cost \/ benefit advantages of the diagnostic device MICROBSCAN<\/span> compared to systems that use standard platforms derive from a research phase that has allowed the development of an innovative technology aimed at improving the performance and robustness of molecular diagnostic techniques.<\/p>\n

The principle that guided the research and design of the MICROBSCAN<\/span> diagnostic system (MS100) was to reach values close to the maximum theoretical sensitivity (a single DNA molecule) regardless of the type of microorganism and \/ or the type of clinical sample in which is contained.<\/p>\n

The high degree of purity of the extracted nucleic acids determines an optimal efficiency of the amplification reactions (PCR \/ RT-PCR) allowing the development of extremely sensitive multiplex tests starting from small quantities of sample.<\/p>\n

MICROBSCAN-KAIROS instrumentation integrates all phases of the analytical process:<\/strong><\/p>\n

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  • Identification of samples by automated bar code reading<\/li>\n
  • Extraction and purification of nucleic acids<\/li>\n
  • Preparation of the PCR plate by introducing the nucleic acids and the reaction mixture containing primers, fluorescent probes, enzymes and internal control<\/li>\n
  • Identification of the pathogen by multiplex qPCR<\/li>\n
  • Interpretation of the analytical signal using a dedicated software that allows the display of the amplification curves, the generation of a final report, the trasmission of the results to the LIS<\/li>\n
  • Quality control of all stages of the process, by means of specific sensors<\/li>\n <\/ul>\n

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    The benefits of the new technology derive from its innovative elements:<\/strong><\/p>\n

    New microfluidic extraction cell<\/span> - comprising:<\/p>\n

    a) An upper compartment to contain the sample, the magnetic particles, the reagents necessary for the extraction of nucleic acids and to obtain an efficient removal of the PCR inhibitors;<\/p>\n

    b) A capillary compartment (volume 5-10 \u03bcl), to allow the handling and exact positioning of magnetic particles immersed in a small volume of liquid, to allow the concentration of nucleic acids and their transfer to an analytical instrumentation.<\/p>\n

    To parallelize the process and facilitate its automation, the geometry of the cell was replicated in an orthogonal model (array) to form an extraction cartridge consisting of 24-192 cells. The increase in sensitivity due to the innovative geometry is equal to 1-2 orders of magnitude.<\/p>\n

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    Process automation \/ miniaturization.<\/strong><\/p>\n

    The extraction cartridge has been designed to be inserted into a robotic module that contains all the functional elements necessary for the extraction \/ purification of nucleic acids (liquid dispensing \/ aspiration, temperature control, vibration, variable magnetic fields, etc.).<\/p>\n

    This allowed:<\/p>\n

    a) to increase the degree of purification of nucleic acids (increase of washing cycles)<\/p>\n

    b) reduce the use of disposable tips by 70%<\/p>\n

    c) load a sufficient number of tips for three runs of 96 samples<\/p>\n

    d) load a quantity of washing solutions that allows about 1000 extractions<\/p>\n

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    Scalability \/ modularity. <\/strong><\/p>\n

    The instrumentation can be easily sized by adding additional modules depending on the number of samples that must be analyzed in 24 hours.<\/p>\n

    a) For small routines (up to 144 samples \/ 12 hours): KAIROS-24 instrumentation<\/p>\n

    b) For medium routines (up to 600 samples \/ 12 hours): KAIROS-96 instrumentation<\/p>\n

    c) For medium to large routines (up to 1800 samples \/ 12 hours): KAIROS-384 instrumentation (under development)<\/p>\n

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    Increase of the quality standards of all mechanical and electronic components <\/span>to allow:<\/p>\n

    a) Electromechanical components completely sealed to allow rapid sterilization and avoid the release of contaminating materials<\/p>\n

    b) Increase of the positioning accuracy of the mechanical axes <10-20\u03bcm (absolute positioning system that does not require calibration)<\/p>\n

    c) Components resistant to wear generated by the H24 use of the instruments.<\/p>\n

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    Thanks to these characteristics, a brief check-up is foreseen every 600 sessions (or six months). After 1000 analytical sessions, the instrumentation requires extraordinary maintenance for the preventive replacement of some components.<\/p>\n

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    Sistema Diagnostico MicrobScan L\u2019 elevata efficienza ed i principali vantaggi costo\/beneficio del dispositivo diagnostico MICROBSCAN rispetto a sistemi che utilizzano piattaforme standard derivano da una fase di ricerca che ha consentito di sviluppare una tecnologia innovativa finalizzata al miglioramento delle performance e della robustezza delle tecniche di diagnostica molecolare. Il principio che ha guidato la […]<\/p>","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"page-templates\/full-width-page.php","meta":{"footnotes":""},"class_list":["post-78","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/nurex.it\/en\/wp-json\/wp\/v2\/pages\/78","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nurex.it\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nurex.it\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nurex.it\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nurex.it\/en\/wp-json\/wp\/v2\/comments?post=78"}],"version-history":[{"count":17,"href":"https:\/\/nurex.it\/en\/wp-json\/wp\/v2\/pages\/78\/revisions"}],"predecessor-version":[{"id":2405,"href":"https:\/\/nurex.it\/en\/wp-json\/wp\/v2\/pages\/78\/revisions\/2405"}],"wp:attachment":[{"href":"https:\/\/nurex.it\/en\/wp-json\/wp\/v2\/media?parent=78"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}